Ion conductance microscopy (ICM) is a useful tool for obtaining non-invasive images of cell surface topography. Recently, it is being used for imaging live cells in culture medium. Cell volume is one of the important factors for cell research because the regulation of cell volume is fundamental to cellular homeostatic mechanism. ICM can provide accurate volume measurement of cells due to its ability to acquire quantitative dimension information. Furthermore, it can calculate cell volume changes over time caused by physiological environment or its cell status changes. In this paper, live HEK293T cell was successfully imaged and its volume changes monitored using Park ICM .

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