Principles of Fluorescence Techniques

The course is designed for students who utilize fluorescence instrumentation and techniques and for researchers and industrial scientists who intend to deepen their knowledge of fluorescence techniques and applications.

The theoretical lectures delivered by key scientists in the field are complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies. Topics addressed in this course include:

Basic Definitions and Principles of Fluorescence
Fluorescence Polarization
Time-Resolved Fluorescence
Instrumentation
Data Manipulation and Data Analysis
Confocal and Multiphoton Fluorescence Microscopy
FLIM, Fluorescence Lifetime Imaging
FCS, Fluorescence Correlation Spectroscopy
Single Molecule Imaging
Image processing and Deconvolution Approaches

It is recommended that participants have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending this course.